ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,GRN (granulin) (alternativename:GEP;GP88;PEPI;PGRN;CLN11;PCDGF). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_002087.3
Thefull sequence-verifiedtargetisexpressedwithHAtagatitsC-terminalunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP818
