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Gentarget/h CYP19A1 inducible lentiviral particles/LVP651/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,CYP19A1: cytochromeP450,family19,subfamilyA,polypeptide1. (alternativename:ARO;ARO1;CPV1;CYAR;CYP19;CYPXIX;P-450AROM).Thesequenceis100%matchedCDSregion (NCBIID:NM_000103.3 ).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP651
