ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,CXCL1: chemokine(C-X-Cmotif)ligand1(melanomagrowthstimulatingactivity,alpha) (alternativename:FSP;GRO1;GROa;MGSA;MGSA-a;NAP-3;SCYB1 ). Thesequenceis100%matchedCDSregion (NCBIID:NM_001511 ).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedforinducibleexpressionwithtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP730
