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Gentarget/h CD33 (6His) inducible lentiviral particles/LVP1095/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,CD33 (human CD33molecule ) (alternativename:p67;SIGLEC-3;SIGLEC3). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_001772.3
Thefull sequence-verifiedtargetisexpressedwithC-terminal6Histagunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP1095
