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Gentarget/h CD274 / PDL1 (6His) inducible lentiviral particles/LVP1077/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,CD274 (humanCD274molecule) (alternativename:B7-H;B7H1;PD-L1;PDCD1L1;PDCD1LG1;PDL1). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_014143.3.
Thefull sequence-verifiedtargetisexpressedwithC-terminal6Histagunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP1077
