Pre-madetetracyclineregulator(TetR)lentiviralparticles expressing sequenceverifiedtetracyclineregulatorgeneunderanenhancedEF1apromoter(whichisnon-tissue,non-celltypespecificpromoter,notbesilenced afterlong-termculture). AblasticidinantibioticMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpression.SeeProductManual (.pdf).
TetR isusedintetracyclineinducIBLeexpression.Itbinds toanyinduciblepromotersthathasincorporateditsbindingsequence torepresstargetexpression.And targetexpressionisinducedoncetetracyclineisadded.TetracyclinebindstoTetR,which releasesTetRfromtarget’spromoter,andstartsthetranscription.Gentarget’sOptionalinducibleshRNAparticlesandOptionalnducibleexpressionparticlescanbeturnedintotetracyclineinduciblesystembyusingTetRexpressingparticles.ByusingadifferentantibioticmarkerinTetRparticlesthanthatinexpressionparticles,youcanselectthedoubletransducedcellsviadoubleantibioticselectionatthesametime.Then,thetarget’sexpressionisturnedintoatetracyclinedosedependentinducibleexpression.
Thisistheblasticidin versionofTetRparticlespurifiedintoPBSsolution.
Amount: 200ul/pervial,at1x107IFU/mlinPBS.
Cat#:LVP459-Bsd-PBS
