Pre-madefireflyLuciferase(luc)Lentiviralparticles expressing luciferaseII geneunder anengineeredEF1apromoterwhich isnon-tissuespecific,highlyexpressedinallcelltypes,andlesslikelybesilencedafterlong-termculture.AGFPMarkerwas co-expressedunderthesamepromoter.LuciferaseandGFPwerebicistronicallyexpressed asindividualproteinmediatedbya2Aelement. ARFP-Puromycinfusiondualmarkerwasexpressedunder RSVpromoter.

Thisisdoublefluorescentlabeledluciferase expressionlentivirusinwhichtheGFPreflectstheluciferaseexpression(alsoformonitoringthesuCMVpromoteractivityinyourcellline),andtheRFPreflectsthepuromycinexpression(alsofor monitoringtheRSVpromoteractivityinyourcellline). ItallowstosortorselecttransducedcellsforlongtermexpressionviaGFPorRFP  signal,aswellasviablasticidinantibioticselection(Dualselection).  GFPsignalprovidesaconvenient,realtimemonitoringthe luciferaseparticlesperformance.  Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at1x10⁷IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP464(Note:thealternativeproduct,Cat#:LVP462,drivenbyanoptionalinducIBLeCMVpromoter).