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Gentarget/EF1a-h Cas9 (RFP-Puro) lentiviral particles/LVP709/1 Ea
Pre-madelentivialparticles expresshumancodonoptimizedwild-type Cas9endonuclease under anenhanced EF1apromoter, withourproprietaryNLS (NuclearLocalizationSignal)atitsN-terminal,forthebestnuclearpenetration.Italsocontainsa RFP-Puromycin fusiondualMarker(underRSVpromoter)forselectionofthetransducedcellsviaRFPfluorescentsignalorviaPuromycinantibiotic.Thefluorescentsignalprovidesareal-timemonitoringofthelentivirus’transductionefficiencyinyourcelltypes.
SeeProductManual here (.pdf).
Application:usedforCRISPRgeneediting(Note:usedwitha separate “genespecificgRNA lentivirus”or”genespecificgRNAvector”forCRISPR editing )
Amount: 5x106IFU/mlx200ulinDMEMmedium with10%FBS,60ug/ml polybrene.
Shippingandstorage: overnightshipmentindry-icepackage.Storedat-80oC.
Cat#:LVP709
