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Gentarget/EF1a-h Cas9 (RFP-Bsd) lentiviral particles, in vivo ready/LVP711-PBS/1 Ea
Pre-madelentivialparticles expresshumancodonoptimizedwild-type Cas9endonuclease under anenhanced EF1apromoter, withourproprietaryNLS (NuclearLocalizationSignal)atitsN-terminal,forthebestnuclearpenetration. Italsocontainsa RFP-lasticidin fusiondualMarker(underRSVpromoter)forselectionofthetransducedcellsviaRFPfluorescentsignalorviaBlasticidinantibiotic.Thefluorescentsignalprovidesareal-timemonitoringofthelentivirus’transductionefficiencyinyourcelltypes.
The particleswasconcentratedandprovidedinPBSsolutionwhichusedforserumsensitiveculture,hardtotransducedcellsorforinvivoapplication.
SeeProductManual here (.pdf).
Application:usedforCRISPRgeneediting(Note:usedwitha separate “genespecificgRNA lentivirus”or”genespecificgRNAvector”forCRISPR editing )
Amount: 5x107IFU/mlx200ulinPBS.
Shippingandstorage: overnightshipmentindry-icepackage.Storedat-80oC.
Cat#:LVP711-PBS
