Pre-madelentivialparticles expresshumancodonoptimizedwild-type Cas9endonuclease under anenhanced EF1apromoter, withourproprietaryNLS (NuclearLocalizationSignal)atitsN-terminal,forthebestnuclearpenetration. Italsocontainsa GFP-Puromycin fusiondualMarker(underRSVpromoter)forselectionofthetransducedcellsviaGFPfluorescentsignalorviaPuromycinantibiotic.Thefluorescentsignalprovidesareal-timemonitoringofthelentivirus’transductionefficiencyinyourcelltypes.
The particleswasconcentratedandprovidedinPBSsolutionwhichusedforserumsensitiveculture,hardtotransducedcellsorforinvivoapplication.
SeeProductManual here (.pdf).
Application:usedforCRISPRgeneediting(Note:usedwitha separate “genespecificgRNA lentivirus”or”genespecificgRNAvector”forCRISPR editing )
Amount: 5x107IFU/mlx200ulinPBS.
Shippingandstorage: overnightshipmentindry-icepackage.Storedat-80oC.
Cat#:LVP708-PBS
