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Gentarget/EF1a Luc（萤火虫）-2A-GFP（RFP-Puro），体内就绪/LVP464-PBS/1 Ea
Pre-madefireflyLuciferase(luc)Lentiviralparticles expressing luciferaseII geneunderanengineeredEF1apromoterwhichisnon-tissuespecific,highlyexpressedinallcelltypes,andlesslikelybesilencedafterlong-termculture.AGFPMarkerwas co-expressedunderthesamepromoter.LuciferaseandGFPwerebicistronicallyexpressed asindividualproteinmediatedbya2Aelement. ARFP-Puromycinfusiondualmarkerwasexpressedunder RSVpromoter.
ThisisdoublefluorescentlabeledluciferaseexpressionlentivirusinwhichtheGFPreflectstheluciferaseexpression(alsoformonitoringthesuCMVpromoteractivityinyourcellline),andtheRFPreflectsthepuromycinexpression(alsoformonitoringtheRSVpromoteractivityinyourcellline).ItallowstosortorselecttransducedcellsforlongtermexpressionviaGFPorRFPsignal,aswellasviablasticidinantibioticselection(Dualselection). GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.
Particleswaspackagedinserum-freemediumwithoutanyhumanoranimalorigincomponents,thenconcentratedandbufferexchangedintoPBSasinvivoreadystatus. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP464-PBS(Note:analternativeproduct,Cat#:LVP462-PBS,drivedbyanoptionalinducIBLeCMVpromoter.)
