Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandthe RFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo. Theproductdonotcontainanyantibioticmarker.
CRE protein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
CREandRFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesiredwhenitsrepressor(TetR)ispresent.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Seeproductmanualfordetails(.pdf).
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene.
Cat#:LVP805
