Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesameenhancedEF1apromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaPuromycinantibioticselection.
CREandRFPare constitutivelyexpressedinhighlevelindependentofcelltypes.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforserum-freeculture or hard-to-transducedcellline, ordireclyused forinvivoapplication.
SeeProductManual fordetails(.pdf).
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP523-PBS
