Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarker,underthesamesuCMVpromoter.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaPuromycinantibioticselection. CREandRFP isconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene. Cat#:LVP338