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Gentarget/CRE-2A-RFP (Neo), CMV lentivirus in PBS/LVP027-PBS/1 Ea
Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarker,underthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ANeomycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaNeomycinantibioticselection. NLS-CREandRFPare constitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-sensitivecelllinesandforinvivoapplication.Seeproductmanualfordetails(.pdf). Amount: >200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP027-PBS
