Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ANeomycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaNeomycinantibioticselection. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. NLS-CREandRFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformanceSeeproductmanualfordetails(.pdf). Amount: >200ul/pervial,at 1x107IFU/mlinDMEMmediumwith10%FBS. Cat#:LVP027
