Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesameenhancedEF1apromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibioticselection. CREandRFPare constitutivelyexpressedinhighlevelindependentofcelltypes.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforserum-freeculture or hard-to-transducedcellline, ordireclyused forinvivoapplication. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP522-PBS
