Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesameenhanceEF1apromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibioticselection. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. CREandRFPareconstitutivelyexpressedinhighlevel independent of celltypes. RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial, 1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene. Cat#:LVP522
