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Gentarget/CRE-2A-RFP (Bsd), CMV lentivirus in PBS/LVP013-PBS/1 Ea
Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibioticselection. NLS-CREandRFPare constitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-sensitivecelllinesandforinvivoapplication. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP013-PBS
