Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibioticselection.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
NLS-CREandRFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformanceSeeProductManualfordetails(.pdf).
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene.
Cat#:LVP013
