Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandthe RFPMarkerunderthesamesuCMVpromoter.Itdoesnotcontainanyantibioticselectionmarker. ThisparticleswasconcentratedandbufferchangedintoPBSsolution,bestsuitableforhard-to-transducedcelllines,serumsensitivecelllinesandinvivoapplications.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-freecell culture, ordirectlyused forinvivoapplication.
SeeProductmanual fordetails(.pdf).
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP805-PBS
