Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesamesuCMVpromoter.ANeomycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaNeomycinantibioticselection.ThisparticleswasconcentratedandbufferchangedintoPBSsolution,bestsuitableforhard-to-transducedcelllines,serumsensitivecelllinesandinvivoapplications. 

CREandGFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  

Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-sensitivecelllinesandforinvivoapplication.

Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at 5x10⁷IFU/mlinPBS. 

Cat#:LVP408-PBS