Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ANeomycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaNeomycinantibioticselection.

Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

NLS-CREandGFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at1x10⁷IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP408