Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesamesuCMVpromoter.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaPuromycinantibioticselection.ThisparticleswasconcentratedandbufferchangedintoPBSsolution,bestsuitableforhard-to-transducedcelllines,serumsensitivecelllinesandinvivoapplications.
CREandGFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
SeeProductManual fordetails(.pdf).
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP407-PBS
