Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesamesuCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaPuromycinantibioticselection.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
NLS-CREandGFPareconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. Seeproductmanualfordetails(.pdf).
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS.
Cat#:LVP407
