Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder theCAGpromoterwhichisthestrongestpromoterinembryonicstem(ES)cells. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.A RFP-Blasticidin fusiondualMarkerwasexpressedunderRSVpromoter,whichallowtoselectthepositivetransducedstablecellsforlongtermculturevia RFP cellsortingorvia antibioticselection.
CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ThisproductconstitutivelyexpresseshighlevelCREenzyme inEScells. Thislentivirus waspurifiedintoPBSsolutionwhichisusedfornon-serumcellcultureandhard-to-transducedcelltypes,ordireclyusedforinvivoapplication.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial, 5x107IFU/mlinPBS. Cat#:LVP577-PBS
