Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder anenhancedEF1apromoterwhichisastrongpromoterindependentoftissueorcelltypes,andlesslikelytobesilencedinlong-termcultureconditions. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.APuromycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviaPuromycinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ThisproductconstitutivelyexpressesnuclearlocalizedCREenzyme inallcelltypes. Thislentivirus waspurifiedintoPBSsolutionwhichisusedfornon-serumcellcultureandhard-to-transducedcelltypes,ordireclyusedforinvivoapplication.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial, 5x107IFU/mlinPBS. Cat#:LVP520-PBS
