Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder anenhancedEF1apromoterwhichisastrongpromoterindependentoftissueorcelltypes,andlesslikelytobesilencedinlong-termcultureconditions. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.APuromycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviapuromycinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ThisproductconstitutivelyexpressesnuclearlocalizedCREenzyme inallcelltypes.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene. Cat#:LVP520
