Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunderinducIBLe suCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo. APuromycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
NLS-CREisconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS. Cat#:LVP339
