Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunderinducIBLe suCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo. ANeomycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic. Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-sensitivecelllinesandforinvivoapplication. NLS-CREisconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at 5x107inPBS. Cat#:LVP297-PBS
