Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunderinducIBLe suCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasetherecombinationeventsinvivo. ANeomycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic.
NLS-CREisconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired. Seeproductmanualfordetails(.pdf). Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. Amount: 200ul/pervial,at 1x107IFU/mlinDMEMmediumwith10%FBS. Cat#:LVP297
