Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder theCAG promoterwhichisthestrongestpromoterinembryonicstem(ES)cells. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.ANeomycinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviaNeomycinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ThisproductconstitutivelyexpressesnuclearlocalizedCREenzyme,speicallyhighlevelin embryonicstem(ES)cells.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene. Cat#:LVP575
