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Gentarget/CRE (GFP-Puro), CAG lentivirus in PBS/LVP576-PBS/1 Ea
Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder theCAGpromoterwhichisthestrongestpromoterinembryonicstem(ES)cells. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.A GFP-Puromycin fusiondualMarkerwasexpressedunderRSVpromoter,whichallowtoselectthepositivetransducedstablecellsforlongtermculturevia GFP cellsortingorvia antibioticselection.
CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
ThisproductconstitutivelyexpresseshighlevelCREenzyme inEScells. Thislentivirus waspurifiedintoPBSsolutionwhichisusedfornon-serumcellcultureandhard-to-transducedcelltypes,ordireclyusedforinvivoapplication.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial, 5x107IFU/mlinPBS. Cat#:LVP576-PBS
