Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunder anenhancedEF1apromoterwhichisastrongpromoterindependentoftissueorcelltypes,andlesslikelytobesilencedinlong-termcultureconditions. CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.AblasticidinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
Thisproductconstitutivelyexpressesa nuclearlocalizedCREenzyme inallcelltypes.
SeeProductManual fordetails(.pdf).
Amount: 200ul/pervial,1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene.
Cat#:LVP519
