Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunderinducIBLe suCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasetherecombinationeventsinvivo. AblasticidinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic.
ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-sensitivecelllinesandforinvivoapplication. NLS-CREisconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired. Seeproductmanualfordetails(.pdf). Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. Amount: 200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP336-PBS
