Pre-madelentiviralparticles expressingsequenceverifiedCRE recombinaseunderinducIBLe suCMVpromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term. This nuclearpermeant CRE increasestherecombinationeventsinvivo.AblasticidinMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpressionviablasticidinantibiotic.
Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
NLS-CREisconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired.
Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS. Cat#:LVP336
