Pre-madetetracyclineregulator(TetR)lentiviralparticles expressing sequenceverifiedtetracyclineregulatorgeneundersuCMVpromoter (forthehighestTetRexpressionlevel). ARFP-Puromycin fusionMarkerunder RSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaPuromycin antibiotic(Dualmarkers).SeeProductmanual(.pdf).
TetR isusedintetracyclineinducIBLeexpression.Itbinds toanyinduciblepromotersthathasincorporateditsbindingsequence torepresstargetexpression.And targetexpressionisinducedoncetetracyclineisadded.TetracyclinebindstoTetR,which releasesTetRfromtarget’spromoter,andstartsthetranscription.Gentarget’sOptionalinducibleshRNAParticlesandOptionalinducibleexpressionparticlescanbeturnedintotetracyclineinduciblesystembyusingTetRexpressingparticles.ByusingadifferentantibioticmarkerinTetRparticlesthanthatinexpressionparticles,youcanselectthedoubletransducedcellsviadoubleantibioticselectionatthesametime.Then,thetarget’sexpressionisturnedintoatetracyclinedosedependentinducibleexpression.
ThisistheRFP-Puromycin dualmaker versionofTetRparticlesinDMEMmediumwith10%FBS.
Amount: 200ul/pervial,at1x107IFU/ml.
Cat#:LVP017-RP
