Pre-madetetracyclineregulator(TetR)lentiviralparticles expressing sequenceverifiedtetracyclineregulatorgeneundersuCMVpromoter (forthehighestTetRexpressionlevel).  AblasticidinantibioticMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpression.SeeProductManual(.pdf).

TetR  isusedintetracyclineinducIBLeexpression.Itbinds toanyinduciblepromotersthathasincorporateditsbindingsequence torepresstargetexpression.And targetexpressionisinducedoncetetracyclineisadded.TetracyclinebindstoTetR,which releasesTetRfromtarget’spromoter,andstartsthetranscription.Gentarget’soptionalinducibleshRNAvectorsandoptionalinducibleexpressionparticlescanbeturnedintotetracyclineinduciblesystembyusingTetRexpressingparticles.ByusingadifferentantibioticmarkerinTetRparticlesthanthatinexpressionparticles,youcanselectthedoubletransducedcellsviadoubleantibioticselectionatthesametime.Then,thetarget’sexpressionisturnedintoatetracyclinedosedependentinducibleexpression.

Thisisthe Puromycin versionofTetRparticlesinDMEMmediumwith10%FBS.

Amount:  200ul/pervial,at1x10⁷IFU/ml. 

 Cat#:LVP017-Puro