Pre-madetetracyclineregulator(TetR)lentiviralparticles expressing sequenceverifiedtetracyclineregulatorgeneundersuCMVpromoter (providingthehighestTetRexpressionlevel). AHygromycinantibioticMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpression.SeeProductManual(.pdf).
TetR isusedintetracyclineinducIBLeexpression.Itbinds toanyinduciblepromotersthathasincorporateditsbindingsequence torepresstargetexpression.And targetexpressionisinducedoncetetracyclineisadded.TetracyclinebindstoTetR,which releasesTetRfromtarget’spromoter,andstartsthetranscription.Gentarget’sOptionalinducibleshRNAparticlesandOptionalinducibleexpressionparticlescanbeturnedintotetracyclineinduciblesystembyusingTetRexpressingparticles.ByusingadifferentantibioticmarkerinTetRparticlesthanthatinexpressionparticles,youcanselectthedoubletransducedcellsviadoubleantibioticselectionatthesametime.Then,thetarget’sexpressionisturnedintoatetracyclinedosedependentinducibleexpression.
ThisistheHygromycin versionofTetRparticlespurifiedintoPBSsolution.
Amount: 200ul/pervial,at1x 108 IFU/mlinPBS.
Cat#: LVP017-Hygro-PBS
