Pre-madetetracyclineregulator(TetR)lentiviralparticles expressing sequenceverifiedtetracyclineregulatorgene undersuCMVpromote (forthehighestTetRexpressionlevel). AHygromycin antibioticMarkerunder RSVpromoter,allowstoselecttransducedcellsforlongtermexpression.SeeProductManual(.pdf).
TetR isusedintetracyclineinducIBLeexpression.Itbinds toanyinduciblepromotersthathasincorporateditsbindingsequence torepresstargetexpression.And targetexpressionisinducedoncetetracyclineisadded.TetracyclinebindstoTetR,which releasesTetRfromtarget’spromoter,andstartsthetranscription.Gentarget’sOptionalinducibleshRNAparticlesandOptionalinducibleexpressionparticlescanbeturnedintotetracyclineinduciblesystembyusingTetRexpressingparticles.ByusingadifferentantibioticmarkerinTetRparticlesthanthatinexpressionparticles,youcanselectthedoubletransducedcellsviadoubleantibioticselectionatthesametime.Then,thetarget’sexpressionisturnedintoatetracyclinedosedependentinducibleexpression.
Thisisthe Hygromycin versionofTetRparticlesinDMEMmediumwith10%FBS.
Amount: 200ul/pervial,at1x107IFU/ml.
Cat#:LVP017-Hygro
