Pre-madeLuciferase(luc)Lentiviralparticles expressing firefly luciferase3 geneunderan optionalinducIBLe suCMV promoter.A GFP Markerwas co-expressedunderthesamepromoter.LuciferaseandGFPwere bicistronicallyexpressed asindividualproteinmediatedbya2Aelement. A RFP-Puromycin fusion antibioticmarker(Dualmarker)wasexpressedunder RSVpromoter. See ProductManual fordetails(.pdf).
Thisis doublefluorescent labeledluciferase expressionlentivirusinwhichthe GFP reflectstheluciferaseexpression(alsoformonitoringthesuCMVpromoteractivityinyourcellline),andthe RFP reflectstheblasticidinexpression(alsofor monitoringtheRSVpromoteractivityinyourcellline). Itallowstosortorselecttransducedcellsforlongtermexpressionvia GFP or RFP signal,aswellasviaPuromycinantibioticselection(Dualselection). GFP signalprovidesaconvenient,realtimemonitoringthe luciferaseparticlesperformance.
ParticleswasconcentratedandbufferexchangedintoPBSsolution.
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP462-PBS
