Pre-madeLuciferase(luc)Lentiviralparticles expressing firefly luciferase3 geneunderan optionalinducIBLe suCMV promoter.A GFP Markerwas co-expressedunderthesamepromoter.LuciferaseandGFPwere bicistronicallyexpressed asindividualproteinmediatedbya2Aelement. A RFP-Puromycinfusion antibioticmarker(Dualmarker)wasexpressedunder RSVpromoter. See ProductManual fordetails(.pdf).

Thisis doublefluorescent labeledluciferase expressionlentivirusinwhichthe GFP reflectstheluciferaseexpression(alsoformonitoringthesuCMVpromoteractivityinyourcellline),andthe RFP reflectstheblasticidinexpression(alsofor monitoringtheRSVpromoteractivityinyourcellline). Itallowstosortorselecttransducedcellsforlongtermexpressionvia GFP or RFP  signal,aswellasviaPuromycinantibioticselection(Dualselection).  GFP signalprovidesaconvenient,realtimemonitoringthe luciferaseparticlesperformance. 

Amount:  200ul/pervial,at1x10⁷IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP462