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Gentarget/CMV-Luciferase (firefly)-2A-GFP (RFP-Bsd)/LVP461/1 Ea
Pre-madeLuciferase(luc)Lentiviralparticles expressing firefly luciferase3 geneunderan optionalinducIBLe suCMV promoter.A GFP Markerwas co-expressedunderthesamepromoter.LuciferaseandGFPwere bicistronicallyexpressed asindividualproteinmediatedbya2Aelement. ARFP-Blasticidinfusion antibioticmarker(Dualmarker)wasexpressedunder RSVpromoter. See ProductManual fordetails(.pdf).
Thisisdoublefluorescentlabeledluciferase expressionlentivirusinwhichtheGFPreflectstheluciferaseexpression(alsoformonitoringthesuCMVpromoteractivityinyourcellline),andtheRFPreflectstheblasticidinexpression(alsofor monitoringtheRSVpromoteractivityinyourcellline). ItallowstosortorselecttransducedcellsforlongtermexpressionviaGFPorRFP signal,aswellasviablasticidinantibioticselection(Dualselection). GFPsignalprovidesaconvenient,realtimemonitoringthe luciferaseparticlesperformance.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBS.
Cat#:LVP461
