GentargetprovidesdifferentsetsofpremadelentivirialparticlesforhumanormouseiPSgenes(seeiPSparticleproductpage). ThispremadelentiviralparticlesisthesinglevectormethodforiPScellgeneration.FourhumaniPSfactor,c-Myc,Klf4,Oct4andSox2(MKOS)wereclonedunderthesameenhanced EF1apromoter.All4factorswereclonedinframebutseparatedby2Asignalfragment,whichresultintheexpressionoffourindividualfactorvia"ribosomalskipping"mechanism.ThisparticlesalsocontainsaPuromycin antibioticMarkerunderRSVpromoter,whichprovides aselctiontoolviapuromycinantibiotic. Thisparticlewasproducedbyco-transfectionofthechainediPSinducIBLelentivectorwithapackagingmix(Cat#HT-pack)intoa293Tpackagingcells(cat#TLV-C).Thepre-madelentiviralparticlesareVSV-Gpseudotypedvirus,packagedin10%FBSmedium,andsuppliedas500ul/pervialat~1x106IFU/ml. Seeproductmanual(.pdf)fordetails. Amounts: hMKOS: 0.5ml/tubetubes, at1x106IFU/ml Cat#:LVP358-Puro
