>
产品中心 >
Target_Expression >
Gentarget/CFP-Annexin5 (Puro) fusion lentiviral particles/LVP445-C/1 Ea
Pre-madelentiviralparticlesexpressingafusiontargetof(CFP-Annexin5). ThefulllengthumanAnnexin5-gene (NCBIaccessionID:NM_001154 ) wasfusioned inframewithCFPatitsN-terminal,expressedunderaconstitutivesuCMVpromoter,and containingapuromycin antibiotic Markerunderthe RSVpromoter.
FluorescentlabelledAnnexin5(or AnnexinV)canbeusedtodetectearlyapoptoticcells.Annexin5interactsstronglyandspecificallywithphosphatidylserine(PS)thatexposuredfromplasmamembraneintheearlyeventinapoptosis.Therefore,Annexin5canbeusedtodetectapoptosisbytargetingforthelossofplasmamembraneasymmetry.LabeledannexinVcanbeappliedbothinflowcytometryandinlightmicroscopyinlivingcultureculture.
Pleaseseeproductmanualfordetails.
Amount:200ul/vial(1×108IFU/ml), providedinDMEMmediumwith10%FBSand60ug/mlPolybrene(10x).
Cat#:LVP445-C
